Learn How CHT™ Media Can Maximize Purity and Recovery in Downstream Processing

December 7, 2015
For more information, visit http://www.bio-rad.com/CHTresins This whiteboard animation video provides a brief introduction to Bio-Rad’s innovative mixed-mode chromatography media, CHT Ceramic Hydroxyapatite. The unique separation properties and selectivity of CHT Media enable you to maximize purity and recovery in a single step of downstream purification for the development of biotherapeutics. CHT Ceramic Hydroxyapatite is a versatile chromatography support used for the separation of biological molecules including polyclonal and monoclonal antibodies, antibody fragments, enzymes, nucleic acids, and membrane proteins. CHT is a spherical, macroporous form of hydroxyapatite. This mixed-mode support has functional groups consisting of pairs of positively charged crystal calcium ions, or C-sites, and clusters of six negatively charged oxygen atoms associated with triplets of crystal phosphates, or P-sites. Bio-Rad's innovative mixed-mode CHT Media offer unique separation properties and unparalleled selectivity and resolution for a variety of molecules. These media can be used at any stage in a process from initial capture to final polishing. Two types of CHT media are available in three particle sizes (20, 40, and 80 µm): • Type I has a higher protein binding capacity and better capacity for acidic proteins • Type II has a lower protein binding capacity but offers better resolution of nucleic acids and certain proteins; it also has a very low affinity for albumin and is particularly well-suited for the purification of many species and classes of immunoglobulins Bio-Rad Process Separations offers a full range of chromatography solutions for process-scale biomolecule purifications. Visit us on the Web to learn more. http://www.bio-rad.com/en-us/category/cht-ceramic-hydroxyapatite-crystalline-hydroxyapatite-resins?WT.mc_id=sm-PPD-ww-cht_vyt_20151202-sidfEb4Jl6c We Are Bio-Rad. Our mission: To provide useful, high-quality products and services that advance scientific discovery and improve healthcare. At Bio-Rad, we are united behind this effort. These two objectives are the driving force behind every decision we make, from developing innovative ideas to building global solutions that help solve our customers' greatest challenges. Connect with Bio-Rad Online: Website: http://www.bio-rad.com/ LinkedIn: https://www.linkedin.com/company/1613226/ Facebook: https://www.facebook.com/biorad/ Twitter: https://twitter.com/BioRadLifeSci Instagram: @BioRadLabs Snapchat: @BioRadLabs

No Previous Videos

Next Video
Optimization to Automation: The Advantages of Multidimensional (Multi-D) Chromatography
Optimization to Automation: The Advantages of Multidimensional (Multi-D) Chromatography

For more information, visit: http://www.bio-rad.com/yt/3/Multi-D In this webinar, Philip Chapman of Bio-Rad...